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Title: Demonstration of Nitric Oxide Synthase Activity in Crustacean Hemocytes and Anti-microbial Activity of Hemocyte-derived Nitric Oxide
Authors: Yeh, F. C.;S.-H. Wu;C. Y. Lai;C. Y. Lee
Contributors: 生物系
Keywords: Bactericidal activity;Crustacean;Hemocyte;Lipopolysaccharide;Nitric oxide;Nitric oxide synthase
Date: 2006-05
Issue Date: 2011-05-16T07:39:46Z
Publisher: Elsevier Science
Abstract: We determined the biochemical characteristics of nitric oxide synthase (NOS) in hemocytes of the crayfish Procambarus clarkii and investigated the roles of hemocyte-derived NO in host defense. Biochemical analysis indicated the presence of a Ca2+-independent NOS activity, which was elevated by lipopolysaccharide (LPS) treatment. When bacteria (Staphylococcus aureus) and hemocytes were co-incubated, adhesion of bacteria to hemocytes was observed. NO donor sodium nitroprusside (SNP) significantly increased the numbers of hemocytes to which bacteria adhered. Similarly, LPS elicited bacterial adhesion and the LPS-induced adhesion was prevented by NOS inhibitor NG-monomethyl-l-arginine (l-NMMA). Finally, plate count assay demonstrated that addition of LPS to the hemocytes/bacteria co-incubation resulted in a significant decrease in bacterial colony forming unit (CFU), and that l-NMMA reversed the decreasing effect of LPS on CFU. The combined results demonstrate the presence of a Ca2+-independent LPS-inducible NOS activity in crayfish hemocytes and suggest that hemocyte-derived NO is involved in promoting bacterial adhesion to hemocytes and enhancing bactericidal activity of hemocytes.
Relation: Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 144(1):11-17
Appears in Collections:[生物技術研究所] 期刊論文

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