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Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/8997

Title: Microbial Community Structure During Oxygen-stimulated Bioremediation in Phenol-contaminated Groundwater
Authors: Lin, C. W.;C. Y. Lai;L. H. Chen;W. F. Chiang
Contributors: 生物系
Keywords: 16S rDNA;Groundwater;Microbial community;Phenol;Remediation;SSCP
Date: 2007-02
Issue Date: 2011-05-16T07:39:47Z
Publisher: Elsevier Science
Abstract: This research explored the changes in genetic diversity and spatial distribution of microbial communities in association with the changes in phenol concentration during a bioremediation process. Results using the traditional plate count method indicated an increase of average bacteria densities in groundwater from 104 to 107 CFU ml-1 initially to 107 to 109 CFU ml-1 after remediation. The diversity and stability of phenol-degrading bacterial communities were investigated by using single-strand-conformation polymorphism (SSCP) genetic profile analysis of 16S rDNA fragments amplified from groundwater samples. The molecular data showed a high degree of genetic similarity between communities from certain monitoring wells during the early phases of remediation, probably due to similar initial physical conditions among wells. Molecular signatures of several cultivated phenol-degrading bacterial strains could be seen in most groundwater profiles throughout the study period, suggesting that these strains were indigenous to the study site. It was also observed that the species diversity of these microbial communities increased as the phenol levels in the groundwater decreased during the 9-month study period, and recovered to the pre-treatment levels after the remediation program was completed.
Relation: Journal of Hazardous Materials, 140(1/2):221-229
Appears in Collections:[生物技術研究所] 期刊論文

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