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Title: Characterization of the Pattern of Cytokeratin Proteins in the Epidermal Cells of Loach, Misgurnus Anguillicaudatus
Authors: Tsai, Jong-Chang
Contributors: 應用運動科學所
Keywords: cytokeratin;epidermis;loach;monoclonal antibody;immunohistochemistry;immunoblotting;cell separation;cell differentiation
Date: 1996-08
Issue Date: 2012-04-20T02:20:39Z
Publisher: Springer Verlag
Abstract: The pattern of cytokeratin proteins in the epidermal cells of loach was studied by immunotechniques and partial separation of the epidermal cells. Two monoclonal antibodies, namely 8F7 and 1C45, against the cytokeratin proteins of the loach epidermis were prepared. these two monoclonal antibodies exhibit distinctive results in immunohistochemical staining. The 8F7 monoclonal antibody stains mainly with the epithelial cells, while the 1C45 monoclonal antibody stains specifically with the club cells. The pattern of cytokeratin proteins in the club cells and the epithelial cells of various epidermal layers was further determined by partial separation of these cells. Immunoblotting analysis of the cell fractions confirms the cytokeratin proteins to be differentially expressed in the club cells and the epithelial cells. However, the cytokeratin proteins expressed in the epithelial cells of the basal, middle and outer layers are same. The results indicate that differentiation of the epithelial cells seems limited during their translocation from basal to upper layers, but in those cells that do differentiate into club cells, the cytokeratin pattern changes.
Relation: Fish Physiology & Biochemistry, 15(4): 307-316
Appears in Collections:[應用運動科學研究所] 期刊論文

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