National Changhua University of Education Institutional Repository : Item 987654321/9753
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 6491/11663
造访人次 : 25204893      在线人数 : 90
RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 进阶搜寻

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.ncue.edu.tw/ir/handle/987654321/9753

题名: Characterization of the Pattern of Cytokeratin Proteins in the Epidermal Cells of Loach, Misgurnus Anguillicaudatus
作者: Tsai, Jong-Chang
贡献者: 應用運動科學所
关键词: cytokeratin;epidermis;loach;monoclonal antibody;immunohistochemistry;immunoblotting;cell separation;cell differentiation
日期: 1996-08
上传时间: 2012-04-20T02:20:39Z
出版者: Springer Verlag
摘要: The pattern of cytokeratin proteins in the epidermal cells of loach was studied by immunotechniques and partial separation of the epidermal cells. Two monoclonal antibodies, namely 8F7 and 1C45, against the cytokeratin proteins of the loach epidermis were prepared. these two monoclonal antibodies exhibit distinctive results in immunohistochemical staining. The 8F7 monoclonal antibody stains mainly with the epithelial cells, while the 1C45 monoclonal antibody stains specifically with the club cells. The pattern of cytokeratin proteins in the club cells and the epithelial cells of various epidermal layers was further determined by partial separation of these cells. Immunoblotting analysis of the cell fractions confirms the cytokeratin proteins to be differentially expressed in the club cells and the epithelial cells. However, the cytokeratin proteins expressed in the epithelial cells of the basal, middle and outer layers are same. The results indicate that differentiation of the epithelial cells seems limited during their translocation from basal to upper layers, but in those cells that do differentiate into club cells, the cytokeratin pattern changes.
關聯: Fish Physiology & Biochemistry, 15(4): 307-316
显示于类别:[應用運動科學研究所] 期刊論文

文件中的档案:

档案 大小格式浏览次数
2070100410001.pdf230KbAdobe PDF583检视/开启


在NCUEIR中所有的数据项都受到原著作权保护.

 


DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈