National Changhua University of Education Institutional Repository : Item 987654321/9756

English | 正體中文 | 简体中文 | Items with full text/Total items : 6507/11669
Visitors : 30005738 Online Users : 347

RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.

Scope Adv. Search

Login ‧ Upload ‧ Help ‧ About ‧ Administer

NCUEIR > College of Social Science and Physical Education > ss > Periodical Articles > Item 987654321/9756

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/9756

Title:	The Wheat Germ Agglutinin Binding Sites and Development of the Mitochondria-rich Cells in Gills of Tilapia (Oreochromis mossambicus)
Authors:	Tsai, Jong-Chang;Hwang, P. P.
Contributors:	應用運動科學所
Keywords:	mitochondria-rich cell, wheat germ agglutinin, bromo-deoxyuridine, calcium, gill, fish
Date:	1998-07
Issue Date:	2012-04-20T02:21:14Z
Publisher:	Springer Verlag
Abstract:	By labelling with gold-colloid-conjugated wheat germ agglutinin (WGA), the WGA-binding sites were identified in the apical region of certain mitochondria-rich (MR) cells in gills of freshwater-adapted tilapia (Oreochromis mossambicus). Bromo-deoxyuridine (BrdU) injection was used to discern the differentiation of the WGA binding sites during development of MR cells. Double labeling of gill sections of fish 2, 4, 6, and 8 days after a single BrdU injection showed that the BrdU labeled MR cells 2 and 4 days after injection were mostly WGA negative, whereas 6 and 8 days after injection, WGA and BrdU-labeled MR cell increased gradually in number. Furthermore, the ratio of the WGA-positive to total MR-cells was higher in gills of tilapia adapted to low calcium ([Ca] = 0.015 mm) freshwater than in hard freshwater ([Ca] = 1.898 mm). The results indicate that WGA binding site may be a marker expressed late during differentiation of MR cells, and physiologically functional in assisting uptake of calcium when environmental calcium is low. According to their WGA binding and also characteristics their likely function in calcium absorption, the WGA positive MR cells shown in this study are considered to be similar to the cells described by Pisam et al. (1995).
Relation:	Fish Physiology & Biochemistry, 19(1): 95-102
Appears in Collections:	[ss] Periodical Articles

Files in This Item:

File	Size	Format
2070100410007.pdf	135Kb	Adobe PDF	880	View/Open

Loading...