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http://ir.ncue.edu.tw/ir/handle/987654321/9757
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| Title: | Isoform expression of Na1-K1-ATPase a-subunit in gills of the teleost Oreochromis mossambicus |
| Authors: | Lee, T. H.;Tsai, Jong-Chang;Yiu, M. J.;Fang, M. J.;Hwang, P. P. |
| Contributors: | 應用運動科學所 |
| Keywords: | Isoform expression |
| Date: | 1998-09
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| Issue Date: | 2012-04-20T02:21:26Z
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| Publisher: | American Physiological Society |
| Abstract: | Three isoform-specific antibodies, 6F against the α1-isoform of the avian sodium pump, HERED against the rat α2-isoform, and Ax2 against the rat α3-isoform, were used to detect the expression of Na+-K+-ATPase α-subunits in gills of a teleost, the tilapia (Oreochromis mossambicus). Tilapia gill tissue showed positive reactions to antibodies specific for α1- and α3-isoforms. The results of immunoblots were converted to numerical values (relative intensities) by image analysis for comparisons. Relative amounts of α1-like isoform alone and consequently the ratio of α1-like to α3-like isoforms were higher in gills of seawater-adapted tilapia than in those of freshwater-adapted ones, indicating that the two isoforms respond differently to environmental salinities. In the subsequent immunocytochemical experiments, gill mitochondria-rich cells were demonstrated to immunoreact with antibodies specific for α1- and α3-isoforms. α1-like and α3-like isoforms of gill Na+-K+-ATPase are suggested to be involved in the ion- and osmoregulation mechanisms in tilapia. Moreover, differential expressions of two isoforms may be associated with different functions, secretion and uptake of ions and acid-base regulation, in gills of seawater- and freshwater-adapted tilapia. |
| Relation: | American Journal of Physiology- Regulatory, Integrative and Comparative Physiology, 275: R926-R932 |
| Appears in Collections: | [應用運動科學研究所] 期刊論文
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| 2070100410008.pdf | 271Kb | Adobe PDF | 1144 | View/Open |
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