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题名: Isoform expression of Na1-K1-ATPase a-subunit in gills of the teleost Oreochromis mossambicus
作者: Lee, T. H.;Tsai, Jong-Chang;Yiu, M. J.;Fang, M. J.;Hwang, P. P.
贡献者: 應用運動科學所
关键词: Isoform expression
日期: 1998-09
上传时间: 2012-04-20T02:21:26Z
出版者: American Physiological Society
摘要: Three isoform-specific antibodies, 6F against the α1-isoform of the avian sodium pump, HERED against the rat α2-isoform, and Ax2 against the rat α3-isoform, were used to detect the expression of Na+-K+-ATPase α-subunits in gills of a teleost, the tilapia (Oreochromis mossambicus). Tilapia gill tissue showed positive reactions to antibodies specific for α1- and α3-isoforms. The results of immunoblots were converted to numerical values (relative intensities) by image analysis for comparisons. Relative amounts of α1-like isoform alone and consequently the ratio of α1-like to α3-like isoforms were higher in gills of seawater-adapted tilapia than in those of freshwater-adapted ones, indicating that the two isoforms respond differently to environmental salinities. In the subsequent immunocytochemical experiments, gill mitochondria-rich cells were demonstrated to immunoreact with antibodies specific for α1- and α3-isoforms. α1-like and α3-like isoforms of gill Na+-K+-ATPase are suggested to be involved in the ion- and osmoregulation mechanisms in tilapia. Moreover, differential expressions of two isoforms may be associated with different functions, secretion and uptake of ions and acid-base regulation, in gills of seawater- and freshwater-adapted tilapia.
關聯: American Journal of Physiology- Regulatory, Integrative and Comparative Physiology, 275: R926-R932
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